CD28^neg population primed by DCs matured with either TLR or cytokines contains a higher proportion of granzyme-expressing, Melan-A specific CD8⁺ T cells. T cells primed by the indicated DCs were stained for the denoted surface molecules and then permeabilized for intracellular staining of granzyme A and B 9 days after priming. The expression of granzyme A and/or B is shown on the x-axis and bars indicate the mean percentage of Melan-A pentamer⁺/ CD8⁺ T cells expressing the granzymes (± SEM). Intracellular granzyme expression was evaluated in Melan-A pentamer⁺/CD8⁺ T cells segregated into CD28⁺ (A) or CD28^neg (B) populations. Boxes indicate the interquartile range (± SEM) and points indicate individual priming wells. Data are combined from 2 donors generated in independent experiments and a total of 11 individual priming wells per condition. Statistical analysis showed no significant differences between groups.