American Society of Hematology

Figure 7

$Figure 7. Rescue of developmental defects in K43M mice by KO of CD25. (A) Flow cytometric analysis of CD25 expression in DN thymocytes. (B) Flow cytometric analysis of LSK fractions from BM. (C) Flow cytometric analysis of DN3 (CD44−CD27−) and DN4 (CD44−CD27+) populations in DN thymocytes. (D) Total number of thymocytes from 3- to 4-week-old mice. The cell numbers are mean ± SE (N = 3 for all the animals). *P < .05, significantly different from the WT control levels. +P < .05, significantly different from the K43M levels. (E) The absolute cell numbers for the LSK populations were calculated based on the percentage of LK (supplemental Table 5) and LSK (supplemental Table 5) in the total cell population from BM (supplemental Table 5). Absolute LSK = total BM cells × LK (%) × LSK (%), expressed as mean ± SE (N = 3). *P < .05, significantly different from the WT controls. +P < .05, significantly different from the K43M levels. (F) Quantification of DN3 cells. The bar graph summarizes the percentage of DN3 (CD44−CD27−) populations from 3 separate experiments. Data are mean ± SE. *P < .05, significantly different from the WT control levels, which were arbitrarily defined as 1 unit (100%). +P < .05, significantly different from the K43M levels.$

Rescue of developmental defects in K43M mice by KO of CD25. (A) Flow cytometric analysis of CD25 expression in DN thymocytes. (B) Flow cytometric analysis of LSK fractions from BM. (C) Flow cytometric analysis of DN3 (CD44⁻CD27⁻) and DN4 (CD44⁻CD27⁺) populations in DN thymocytes. (D) Total number of thymocytes from 3- to 4-week-old mice. The cell numbers are mean ± SE (N = 3 for all the animals). *P < .05, significantly different from the WT control levels. ⁺P < .05, significantly different from the K43M levels. (E) The absolute cell numbers for the LSK populations were calculated based on the percentage of LK (supplemental Table 5) and LSK (supplemental Table 5) in the total cell population from BM (supplemental Table 5). Absolute LSK = total BM cells × LK (%) × LSK (%), expressed as mean ± SE (N = 3). *P < .05, significantly different from the WT controls. ⁺P < .05, significantly different from the K43M levels. (F) Quantification of DN3 cells. The bar graph summarizes the percentage of DN3 (CD44⁻CD27⁻) populations from 3 separate experiments. Data are mean ± SE. *P < .05, significantly different from the WT control levels, which were arbitrarily defined as 1 unit (100%). ⁺P < .05, significantly different from the K43M levels.

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