Cytokine production in β₂GPI–stimulated DC culture supernatants. Five-day human DCs were stimulated with LPS (100 ng/mL), G-β₂GPI (10 μg/mL), M-β₂GPI (10 μg/mL), glucose (250mM), or mannitol (250mM), in the presence or absence of the anti-RAGE mAb (25 μg/mL). Supernatants were collected after 18 hours to measure IL-12 p70, TNF-α, IL-10, and IL-1β by specific ELISA experiments. G-β₂GPI and M-β₂GPI triggered statistically significant up-regulation of all cytokine secretion. Pretreatment of iDCs with saturating concentrations of the blocking anti-RAGE mAb prevented the up-regulation of all cytokines tested in response to G- β₂GPI. For IL-12p70: *P < .001, ^†P < .05, and ^‡P < .01 comparing G-β₂GPI vs α-RAGE + G-β₂GPI; for TNF-α and IL-10: *P < .001 and ^†P < .001 comparing G-β₂GPI vs α-RAGE + G-β₂GPI; for IL-1β: *P < .001, ^†P < .05, and ^‡P < .001 comparing G-β₂GPI vs M-β₂GPI and ^§P < .001 comparing G-β₂GPI vs α-RAGE + G-β₂GPI.