miR-34a directly targets FoxP1 in DLBCL. (A) U2932 cells were electroporated with pre-miR34a or a scrambled negative control pre-miR and analyzed with respect to FoxP1 expression 48 hours later. FoxP1 transcript levels were normalized to GAPDH expression. (B) FoxP1 protein levels of the experiment described in panel A were analyzed by Western blot. α-tubulin levels are shown to control for equal loading. (C) Dual luciferase assay of HEK293T cells cotransfected with firefly luciferase constructs containing the wild-type or mutant miR-34a target site of the FOXP1 3′-UTR downstream of the luciferase reporter. Cells were cotransfected with either pre–miR-34a or a negative control scrambled oligonucleotide and the respective luciferase construct. Data are represented as relative luciferase activity. All experiments were reproduced ≥ 3 times. Error bars indicate SD.