IL-7⁺ and GAL1⁺ stromal cells constitute distinct stromal cell populations. BM sections of IL-7-GFP reporter mice were stained with anti-GAL1, anti-GFP antibodies, and Sytox Blue (nuclei) as indicated and analyzed by confocal microscopy. (A) GFP⁺ IL-7–expressing stromal cells and GAL1⁺ cells are scattered in the BM (original magnification 40×/1.25 NA oil objective). Bar represents 50 μm. (B) Representative IL-7⁺ spindle-shaped stromal cell (original magnification 63×/1.4 NA oil objective). Bar represents 10 μm. Images acquired on Leica SP5X and analyzed with ImageJ 1.44.