Relative SDF-1α protein expression and Bv8 mRNA expression in LLC tumors after treatment with PTX or PTX in combination with either G-CSF or AMD3100. (A) C57Bl/6 mice bearing 500 mm³ LLC tumors (n = 4-5 mice per group) were treated with PTX or PTX in combination with either G-CSF or AMD3100. Tumors were removed 3 days later and sections evaluated for SDF-1α expression (green; scale bar = 500 μm). (B) Quantification of relative SDF-1α expression is provided. (C) In parallel, mRNA was extracted from the same tumors, and Bv8 mRNA levels were measured by qRT-PCR. ***P < .001 from control group, unless indicated otherwise.