Evaluation of tumor cell proliferation and apoptosis in LLC tumors after treatment with PTX or PTX in combination with either G-CSF or AMD3100. (A) C57Bl/6 mice bearing 500 mm3 LLC tumors (n = 4-5 mice per group) were treated with PTX or PTX in combination with either G-CSF or AMD3100. Tumors were removed 3 days later and evaluated for proliferation (red) and apoptosis (green; scale bar = 200 μm). (B) Quantification of perfusion and hypoxia is expressed as percentage. (C) EMT/6, MDA-MB-231, and LLC cells were cultured in the presence of G-CSF or AMD3100 with or without CoCl2. Cell proliferation was evaluated quantitatively with the metabolic indicator dye alamarBlue. Results are presented as the percentage of alamarBlue reduction and were corrected to background values of negative controls.
