Analysis of VEGF-A, and MMP-2 levels in tumors after treatment with PTX, PTX and G-CSF or PTX and AMD3100. (A,C) C57Bl/6 mice bearing 500 mm³ LLC tumors (n = 3 mice per group) or (B,D) BALB/c mice bearing 500 mm³ EMT/6 tumors (n = 3 mice per group) were treated with PTX or PTX in combination with either G-CSF or AMD3100. Tumors were removed 3 days later and tumor lysates were evaluated for (A-B) MMP-2 expression by zymography, and (C-D) VEGF-A expression by ELISA. Summary of data are presented in graphs.