PKCζ expression in LCLs from different donors. The groups of donors were compared: MM/MGUS patients with (P1-P3) and without (P4-P6) paratarg-7 specificity of their paraprotein and healthy donors (HD1-HD5). (A) Quantitative RT-PCR for PKCζ, GAPDH, and PI3K was done as described. Values were normalized for GAPDH. (B) Immunodetection of PKCζ in 5 patients carrying hyperphosphorylated paratarg-7 (P1-5) and 2 healthy donors (HD1-2) carrying wild-type paratarg-7. (C) IEF analysis of these same samples.