ADAM10 activates the TNFα/NFκB axis. (A) ELISA showing a significant down-regulation of TNFα in Jeko-1 cells 48 hours after the transfection of ADAM10 siRNA compared with cells transfected with scrambled siRNA. (B) Western blots showing a down-regulation of phospho-NFκBp65 and total NFκBp65 after ADAM10 down-regulation using siRNA, both in the total lysates harvested from Jeko-1 and Mino cells. (C) Western blots showing a down-regulation of total NFκBp65 in nuclear extracts from Jeko-1 and Mino cells. (D) Using a NFκB reporter vector dual luciferase assay, we showed a significant down-regulation of the NFκB transcriptional activity in Mino cells after down-regulation of ADAM10 using siRNA. (E) Using a NFκB reporter vector dual luciferase assay, we showed a significant up-regulation of the NFκB transcriptional activity in Mino cells after the addition of human recombinant ADAM10 (100 ng/mL).