TORC1/TORC2 signaling in activated lymphocytes. (A) Schematic representing a simplified signaling pathway for TORC1/TORC2 (using ScienceSlides objects, Suite 2009). The dotted green line indicates that the signaling is not direct. (B) AKT and p70S6K phosphorylation in LPS-stimulated (48 hours) cells from spleens and LNs of KI versus WT mice. p-p70S6K levels remained low, as in unstimulated cells, whereas pAKT levels increased. (C) AKT^Ser473 phosphorylation in LPS-stimulated (48 hours) B220⁺ lymphocytes from spleens, LNs, and BM increased in KI mice compared with WT. (D) Time course (0, 15, and 30 minutes) of pAKT^Ser473 induction in splenocytes stimulated with LPS. (E) pAKT^Ser473 levels in control/untreated L363 myeloma cells versus cells treated with 10nM rapamycin for 24 hours. (F) ILK and DNA-PKcs in B220⁺ splenocytes stimulated with LPS after 24 hours. (G) pAKT^Ser473 induction (48 hours) in cells pretreated for 2 hours with 20μM of the DNA-PKcs inhibitor NU7026 and then stimulated with LPS.