Detection of blood lymphocytes and B-cell development in the BM. (A) Top panel: B (B220⁺) and T (CD3⁺) cells detected in the peripheral blood of Rag-2^−/−γ_c^−/− mice reconstituted with Glce^+/+ and Glce^−/− FLHSCs at 4 weeks after transplantation; representative mice are shown. Scatter plot represents individual mice measured and the mean B-/T-cell ratios (n ≥ 10). **P < .01. Bottom panel: Expression of IdoA containing HS on the cell surface of B220⁺ B cells was measured. Bars represent mean ± SEM (n = 4). The dotted black line indicates background staining. **P < .01. (B) BM of Glce^+/+ and Glce^−/− mice was isolated and stained for B220, CD117 (c-Kit), and IgM. The dot plots show 3 distinct B220⁺ B-cell populations; pro-B cells (Pro, CD117⁺/IgM⁻), pre-B cells (Pre, CD117⁻/IgM⁻), and immature B cells (Imm, CD117⁻/IgM⁺). Bar diagram represents mean ± SD of each subsets as percentage of the total B-cell population (n = 4).