Glce^−/− mice have a reduced follicular mature B-cell pool. (A) B-cell maturation in the spleen of Glce^+/+ and Glce^−/− mice was analyzed by triple staining with B220, IgM, and IgD. Gates represent: immature B cells (IgM^lo/IgD⁻); T1, transitional 1 B cells (IgM⁺/IgD^low/−); T2, transitional 2 B cells (IgM⁺/IgD^high); FM, follicular mature B cells (IgM^low/IgD^bright). (B) Left panel: Bar diagram showing the total number of splenic B cells in Glce^+/+ and Glce^−/− mice. Right panel: B-cell subsets quantified by FACS analysis, measuring the percentage of all B220⁺ B cells of the total spleen. The 4 different B220⁺ B-cell populations (as in panel A) are shown in a bar graph (right panel). Bars represent mean ± SD of 6 mice and are representative for 3 independent groups. *P < .05. **P < .01. (C) Serial cryosections of the spleens of Glce^+/+ and Glce^−/− stained for B220 and IgD to identify the B cells. T indicates T-cell area; f, B-cell follicle; and rp, red pulp.