GC formation and splenic plasma cells in Glce^+/+ and Glce^−/− mice. (A) GC formation is undisturbed in Glce^−/− mice. Glce^+/+ and Glce^−/− mice were killed after repeated immunization with KLH-TNP (day 56). Serial cryosections of the spleen were stained with anti-B220 and peanut agglutinin, respectively. T indicates T-cell area; f, B-cell follicle; wp, white pulp; and rp, red pulp. (B) Quantification of GCs in the spleen of Glce^+/+ and Glce^−/− mice. GCs were quantified per millimeter squared tissue section (mean ± SEM; n = 4/group). (B) Glce^−/− mice show decreased splenic plasma cell numbers. Number of syndecan-1⁺ plasma cells in the spleen of Glce^+/+ and Glce^−/− mice quantified per millimeter squared (mean ± SEM; n = 4/group). *P < .05.