Figure 6
![Figure 6. Glce deficiency impairs HS interaction with a subset of growth factors/chemokines. Binding of ligands to Glce+/+ and Glce−/− MEFs. MEFs from Glce+/+ and Glce−/− mice were incubated with (A) growth factors (HGF and APRIL) or (B) chemokines (CXCL12α, CXCL13, and CCL21), washed, and the binding was analyzed by FACS. Graphs represent the relative binding, compared with the Glce+/+ MEFs (relative mean fluorescence intensity [MFI] ± SEM; n = 3-5). **P < .01. ***P < .001. ΔR-APRIL indicates point mutant (R231A) that is unable bind to the high-affinity receptor BCMA and TACI.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/23/10.1182_blood-2010-12-325522/4/zh89991172260006.jpeg?Expires=1735516151&Signature=t771jVinMvp5Q8T1fy~Xk2IIqqffCk4NpyGW98NXGRIcWD30KssELpYH0VPmbKo1GA7BYhXzKcoAV6hfzxYWaEg0zq-87~NPOagQMXJQ9QRg0CaR-RohmAaT77tCqqPDMPp2gOLz61wa-8JcslpL2lCNzqyGT-q37EbUaMXA1llC-dep5LWwZspyoaNFoTX9fgB-jjua6QfOKOQBJNzz~SqvTy1-WwF5yd0RDhdHT9lo6IOF814HTUl7i1EHil6djaE5w~fmtJQzFXP3rokCE0nj8PRoU9m-6JLElHQaUUflBbnkX2wDl0QvGO~JyyBmwpDiIKuhu7mfvwicpXrv2Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Glce deficiency impairs HS interaction with a subset of growth factors/chemokines. Binding of ligands to Glce+/+ and Glce−/− MEFs. MEFs from Glce+/+ and Glce−/− mice were incubated with (A) growth factors (HGF and APRIL) or (B) chemokines (CXCL12α, CXCL13, and CCL21), washed, and the binding was analyzed by FACS. Graphs represent the relative binding, compared with the Glce+/+ MEFs (relative mean fluorescence intensity [MFI] ± SEM; n = 3-5). **P < .01. ***P < .001. ΔR-APRIL indicates point mutant (R231A) that is unable bind to the high-affinity receptor BCMA and TACI.
