Tregs and TLR-expressing Tregs are increased in HCQ-treated patients. Unstimulated (A) and env + gag–stimulated (B) total, naive, and activated Treg (%) in HIV-infected INRs at baseline, after 6 months (ms) of HCQ treatment, and 2 months after HCQ interruption. Unstimulated (C) and env + gag–stimulated (D) TLR2⁺ and TLR4⁺ Tregs and LPS-stimulated (E) TLR4⁺ Tregs (percentage) in the same patients. Mean values, SE, and P values are indicated.