Figure 1
Antigen presentation occurs only in the DLNs after intramuscular rHuAd5 immunization. (A) Experimental design where mice were injected intravenously with 5 × 105 CFSE-labeled OT-I CD8+ T cells 6 days after rHuAd5-SIINFEKL-Luc immunization. Tissues were harvested at day 7, and cells were cultured separately in vitro for 72 hours. (B) CFSE profile of OT-I cells isolated from the spleen, distal LNs (axillary, brachial) or DLNs (inguinal, iliac, popliteal) at 7 days after immunization.
