Normoxic DNAse1 hypersensitivity predicts hypoxic binding of HIF. High-stringency HIF-1-binding regions were ranked according to distance upstream/downstream of the nearest TSS (vertical axis). (A) For each HIF-1-binding region, the position of each RCGTG motif, relative to the center of the HIF-binding region, was plotted (horizontal axis). (B) The same analysis was repeated for the subset of RCGTG motifs that coincided with a normoxic DNAse1 hypersensitivity peak (ENCODE consortium dataset 1). (C) Genomic binding of HIF-1α, HIF-2α, and HIF-1β subunits at the egl nine homolog 3 gene locus. The number of reads per 100-bp window and the location of the gene together with the positions of the RCGTG motifs, and DNAse1-hypersensitive RCGTG (DNAse1-hypersensitive site RCGTG) motifs are shown for comparison.