ChIP analysis of histone modifications within the Aiolos promoter in CLL and normal B cells. (A) Schematic representation of the Aiolos locus. CpG island is represented by a gray box, and the arrows indicate the positions of the primer pairs used to analyze chromatin remodeling at Aiolos promoter. (B-F) ChIP analysis of H3K4 dimethylation (B), H3K4 trimethylation (C), H3K9 acetylation (D), H3K27 trimethylation (E), and H3K9 trimethylation (F) in B cells from HD (black bars) and CLL patients (white bars). qPCR was performed with primers a-f. The gray portions of the graphs correspond to the CpG island. The graph shows the average percent immunoprecipitation with SEM for 8 HD and 14 CLL patients (P1, P2, P17-P28) calculated for each position. (G) Quantitative analysis of SMYD3 mRNA levels in 14 HD and 16 CLL patients (P1, P2, P8, P17, P20-P23, P25, P26, and P28-P33). Data are expressed as normalized expression by use of the 2^−ΔCT calculation method (Ct indicates cycle threshold) and β-actin as reference gene. Differences between groups were tested by use of Mann-Whitney U test (Prism5.0c software; *P < .05; **P < .01; ***P < .001).