Defective development of iNKT cells in Elf-1^−/−mice is cell intrinsic. (A-B) BM cells from WT and Elf-1^−/− mice were transferred into irradiated WT or Elf-1^−/− recipients. After 6 weeks, cells were harvested from recipient mice, stained with CD1d/αGalCer tetramer and anti–TCRβ, and analyzed by flow cytometry. (A) Numbers indicate the percentage of tetramer⁺TCRβ⁺ in the spleen of indicated recipients. Data shown are representative of 3 independent experiments using a minimum of 2 recipients per donor genotype. (B) Bar graphs depict the means ± SD for the proportion of iNKT cells in the spleen of indicated recipients (n = 9 for each group; *P < .05; **P < .01). (C) BM cells from Elf-1^−/− or CD45.1⁺ WT congenic mice or mixed BM (1:1) from Elf-1^−/− and WT congenic mice were transferred into irradiated Jα18^−/− recipients. After 6 weeks, cells were harvested from recipient mice, stained with CD1d/αGalCer tetramer and anti–CD45.1, and analyzed by flow cytometry. Numbers in each quadrant indicate the percentage of tetramer⁺CD45.1⁺ cells in the spleen of indicated recipients. Data are representative of 3 independent experiments using a minimum of 2 recipients per donor genotype.