Analysis of neutrophil disappearance and integrin activation in human subjects following G-CSF administration. (A) WBC differential of donor 1 (D1) human leukocyte subsets at designated times after G-CSF administration, showing the disappearance of neutrophils 30 minutes after G-CSF administration. WBC is given as number of leukocytes × 103/μL. Orange represents neutrophils; blue, lymphocytes; purple, monocytes; green, eosinophils; and black, basophils. (B) Flow cytometric staining of human leukocytes using mAb24 (a monoclonal Ab that specifically recognizes the activated leukocyte integrin bound to Mg2+) for D1 over time. Cells were identified as neutrophils, monocytes, or lymphocytes based on size and granularity by flow cytometry. Colors designate that time samples were collected. Leukocytes exposed to PMA were used as a positive control. (C) Absolute neutrophil count of 4 human donors for 90 minutes after G-CSF administration. Neutrophil count is given as number of neutrophils × 103/μL. (D) Percentage change of MFI from baseline of neutrophils stained with mAb24 after G-CSF administration for the same 4 donors.
