Sustained human myeloid differentiation in the humanized CSF-1 mice. (A) Human CD34⁺ cells engrafted CSF1^m/m, CSF1^h/m, and CSF1^h/h mice were bled after 12, 16, and 20 weeks of transplantation. Cells were stained with CD45, CD14, and CD33 human antibodies, and analyzed by flow cytometry. Cells that are human CD45⁺ were pregated and discriminated based on CD14 and CD33 expression. (B) Relative frequencies of human CD45⁺ CD14⁺CD33⁺ cells were plotted. Each group contains n = 10 mice. Each symbol represents an individual mouse; horizontal bars indicate the mean values. Data are representative of 3 independent experiments.