Frequencies of tissue-derived human monocytes/macrophages in the humanized CSF-1 mice.CSF1^m/m, CSF1^h/m, and CSF1^h/h mice were engrafted with human CD34⁺ cells, and 12 weeks after transplantation, mice were killed perfused with PBS. Liver (A), lungs (B), and skin (C) were harvested and single-cell suspensions were prepared. Peritoneal cavity cells (D) were collected by aspirating with PBS. Cells were stained with human CD45, CD14, and CD33 antibodies, and analyzed by flow cytometry. Each symbol represents an individual mouse; horizontal bars indicate the mean values. Data are representative of 3 independent experiments.