Effects of NrasG12D expression on hematopoietic stem cell and myeloid progenitor populations. (A) Flow cytometric analysis was performed on 3 months old F1 WT (open bars) and Mx1-Cre, NrasG12D (solid bars) bone marrow that were stained with a combination of cell surface markers to characterize HSC, MPP, CMP, GMP, and MEP populations. The absolute numbers of cells in each compartment were calculated using an established formula that estimates that 2 femurs and 2 tibiae contain 20% of total nucleated bone marrow cells.23 P values for LSKF+, CMPs, GMPs, and MEPs are .0578, .0166, .0272, and .0665, respectively. (B) BrdU incorporation by hematopoietic cells enriched for myeloid progenitor activity (CMPs, GMPs, and MEPs as sorted in panel A) and for HSCs (LSK as sorted in panel A) from 3-month-old F1 WT (open bars) and Mx1-Cre, NrasG12D (solid bars) bone marrow. P value for LSK is .0596.
