CCR7 is expressed by neutrophils. (A) Membrane CCR7 expression was analyzed in human fresh whole blood neutrophils and in purified human and mouse neutrophils (top histograms). Inserts correspond to dot-plot analysis. Intracellular expression of CCR7 was analyzed in human fresh whole blood and in purified human and mouse neutrophils after permeabilization (bottom histograms). Results are representative of 1 of 5 independent experiments. (B) Bone marrow neutrophils isolated from WT and Ccr7^−/− mice were incubated with PE-labeled anti-CCR7 (white histogram) and control mAbs (gray histogram). Results are representative of 1 of 5 experiments. (C) The expression of CCR7 mRNA was analyzed in mature murine bone marrow neutrophils isolated from WT mice. Cells were not stimulated or stimulated for 3 hours by GM-CSF, GM-CSF + LPS, LPS, GM-CSF + IL-17, CFA, and TAg. RNA integrity and cDNA synthesis were verified by amplifying GAPDH. Results are representative of 1 of 3 experiments.