Analysis of hematopoietic stem and progenitor cell populations in Ews KO and WT mice. (A) Top panel: schematic diagram of gating strategy; freshly isolated BM cells from Ews^−/− and WT littermate control (Ews^+/+) mice were stained with the indicated antibodies and analyzed by flow cytometry. LT-HSC indicates long-term hematopoietic stem cell (defined as Lin⁻Sca1⁺ c-Kit⁺ and CD34⁻); and ST-HSC + MPP, short-term hematopoietic stem cell including multipotent progenitors (defined as Lin⁻Sca1⁺c-Kit⁺ and CD34⁺). CLP indicates common lymphoid progenitors; CMP, common myeloid progenitors; GMP, granulocyte-monocyte progenitors; and MEP, megakaryocyte-erythroid precursor. Bottom panel: the percentage of cells in each group is summarized in the accompanying table. At least 3 pairs of Ews^−/− mice and WT littermate control mice from different dams were analyzed. A representative plot is shown. (B) BM cells from Ews^+/+ (WT) and Ews^−/− (KO) were sequentially gated as shown to quantify CD150⁺CD48⁻Lin⁻Sca1⁺c-Kit⁺ cells. Numbers represent the percentages of CD150⁺CD48⁻Lin⁻Sca1⁺c-Kit⁺ cells in the gated populations. At least 3 pairs of Ews^−/− mice and WT littermate control mice from different dams were analyzed. A representative plot is shown.