MAIT cells express tissue-specific chemokine receptors and are abundant in the liver. (A) Representative staining for indicated activation markers, chemokine receptors, and NK receptors on CD4⁺ (green) or CD8β⁺ (blue) T cells (CD3⁺γδ⁻) and MAIT cells (CD3⁺γδ⁻CD4⁻CD8β^+/−CD161⁺Vα7.2⁺) (red) from blood. Representative of at least 3 donors. (B) Peripheral blood mononuclear cells, intestine, lymph node (LN), and liver triple immunostaining with the indicated antibodies. MAIT cells are triple-stained cells in the right panel. Examples of mono-color layers on a zoomed triple-stained cell are shown on the right. (Bottom panel) Proportion of MAIT (Vα7.2⁺IL-18Rα⁺CD3⁺) in liver, LN, and intestine. Each symbol represents an individual donor. (C) Representative fluorescence-activated cell sorter staining on CD4⁻CD3⁺γδ⁻ lymphocytes from the indicated organs (n > 100, 3, 4, and 2 for peripheral blood lymphocytes (PBLs), intestine, LN, and liver, respectively).