MAIT cells express RORC and display a mixed Th1/Th17 cytokine secretion pattern. (A) IL-10, Granzyme B, IL-2, TNF-α, IL-17, and IFN-γ secretion by sorted CD4 or CD8 memory T cells and MAIT cells after 48 hours of stimulation by anti-CD3 + CD28 or PMA and ionomycin. Dotted line represents detection limit. (B) Representative IL-17 and IFN-γ intracellular staining of CD4, CD8 T, and MAIT cells after 12 hours of PMA and ionomycin stimulation (left panel). Proportion of CD4, CD8 T, and MAIT cells producing IL-17 or IFN-γ (right panel). (C) Quantitative PCR analysis of RORC expression normalized to actin on sorted CD4 or CD8 memory T and MAIT cells. Each symbol represents an individual donor. (D) IL-10, Granzyme B, IL-2, TNF-α, IL-17, and IFN-γ in the 48-hour culture supernatant of monocytes fed or not with bacteria for 3 hours followed or not by addition of sorted autologous MAIT cells.