T_HS cells in early-stage MF are enriched for clonal or oligoclonal T-cell populations. (A) T_HS and low-scatter T cells were isolated from a panel of patients with stage IA-stage IB MF. (B) The preferential presence of T cells expressing particular TCR Vβ subfamilies was analyzed by comparing the fold change in the frequency of each TCR Vβ subfamily in the high- versus low-scatter population (percentage of TCR Vβ high-scatter/percentage of low-scatter). There was a trend toward oligoclonality or clonality in the high-scatter population. The 3 patients shown had identifiable T-cell clones on TCRγ PCR. SSC-H indicates side-scatter height; FSC-H, forward scatter-height.