Figure 2
Figure 2. Blood flow is not required for the initiation of HSC gene expression, but is essential for its maintenance in zebrafish. Expression of runx1 and cmyb at 24 and 36 hpf in control MO (A,C,E), tnnt2aMO (B,D,F) injected embryos. Arrows mark runx1 and cmyb expression in HSCs. (G-J) The maintenance and homing of HSCs were affected in tnnt2a morphants. Expression of cmyb in CHT in control MO (G) and tnnt2aMO (H) injected embryos at 2 dpf. Expression of rag1 in thymus in control MO (I) and tnnt2aMO (J) injected embryos at 4 dpf. (G-H) Arrows indicate CHT. (I-J) Arrows indicate thymus. Anterior is to the left and dorsal is up. CHT, caudal hematopoietic tissue. (K,L) Quantitative RT-PCR analysis of runx1 and cmyb expression in the dissected trunk region of control and tnnt2aMO embryos at 24 and 36 hpf (mean ± SD, t test, *P < .05, n = 3).

Blood flow is not required for the initiation of HSC gene expression, but is essential for its maintenance in zebrafish. Expression of runx1 and cmyb at 24 and 36 hpf in control MO (A,C,E), tnnt2aMO (B,D,F) injected embryos. Arrows mark runx1 and cmyb expression in HSCs. (G-J) The maintenance and homing of HSCs were affected in tnnt2a morphants. Expression of cmyb in CHT in control MO (G) and tnnt2aMO (H) injected embryos at 2 dpf. Expression of rag1 in thymus in control MO (I) and tnnt2aMO (J) injected embryos at 4 dpf. (G-H) Arrows indicate CHT. (I-J) Arrows indicate thymus. Anterior is to the left and dorsal is up. CHT, caudal hematopoietic tissue. (K,L) Quantitative RT-PCR analysis of runx1 and cmyb expression in the dissected trunk region of control and tnnt2aMO embryos at 24 and 36 hpf (mean ± SD, t test, *P < .05, n = 3).

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