klf2a is downstream of tnnt2a in the regulation of artery maturation and maintenance of HSC programming. Expression of runx1 (A-D) and cmyb (E-H) in HSCs in control MO (A,C,E,G) and klf2aMO (B,D,F,H) embryos at 24, 36, and 48 hpf, respectively. (I-J) Expression of rag1 in thymus in control MO (I) and klf2aMO (J) injected embryos at 4dpf. (K-L) Quantitative RT-PCR analysis of runx1 and ephrinB2 expression in the dissected trunk region of control and klf2aMO embryos at 24 and 36 hpf, respectively (mean ± SD, t test, *P < .05, n = 3).