Size determination of MPs. (A) TEM images of healthy blood plasma- and RA SF-derived, isolated MPs. Original magnification × 50 000. Scale bars represent 500 nm. Images were recorded at room temperature using Hitachi 7100 electron microscope equipped with a Megaview II digital camera. (B) Shaded topography AFM images of healthy blood plasma- and RA SF-derived, isolated MPs. Scale bars represent 500 nm. Images were captured at room temperature using a PSIA XE-100 AFM with a XEP1.5 acquisition software. The imaging medium was air. Topography images were processed with XEI 1.6 software using the Sobel Edge Enhancement method. (C) MP size distributions, determined on the basis of TEM and AFM images of 180-180 MPs, are shown by histograms. For the analysis of particles, ImageJ software (Version 1.42q) was used. (D) Analysis of isolated MPs and native biologic samples by DLS. The x-axis is set to logarithmic scale; a(rh) denotes the coefficient of autocorrelation function of the scattered electric field. Arrows indicate peaks corresponding to MPs in the native samples. DLS experiments were carried out at 21°C.
