Responses of Akt3^−/− platelets to other platelet agonists. (A) Washed wild-type (WT) and Akt3^−/− platelets were stimulated with U46619. Platelet aggregation was monitored using a turbidometric aggregometer at 37°C and 1000 rpm stirring speed. Platelet secretion was recorded concomitantly in the presence of luciferin-luciferase agent. Platelet aggregation was also measured after stimulation with (B) collagen (0.8 μg/mL, n = 6, P = .909), (C) ADP (for 2.5μM, n = 4, P = .1646) and (D) botrocetin and VWF (for Botrocetin 1.0 μg/mL, n = 3, P = .837). Quantitation was performed using Student t test.