Figure 3
Figure 3. Subcellular localization of Fmn2 and its effect on cytoskeleton. The GFP-tagged Fmn2 protein was localized in NIH/3T3 cells and (A) tested for its effect shown on the shape of the cells. (B) Plasma membrane labeling with CellMask. (C) Actin labeling with AlexaFluor-555–conjugated phalloidin. (D) α-tubulin labeling with anti–α-tubulin antibody. Images were captured by a laser-scanning confocal microscope (Bio-Rad MRC-1024 ES) mounted on a Nikon TE-300 using a 60×/1.4 NA oil Plan Apo VC objective, digitally acquired using Laser Sharp software Version 3.2 (Bio-Rad), and analyzed using NIH ImageJ Version 1.42l software. Data are representative of 3 independent experiments. The GFP vector alone was used as a control. Ovals and arrows indicate transfected and nontransfected cells, respectively.

Subcellular localization of Fmn2 and its effect on cytoskeleton. The GFP-tagged Fmn2 protein was localized in NIH/3T3 cells and (A) tested for its effect shown on the shape of the cells. (B) Plasma membrane labeling with CellMask. (C) Actin labeling with AlexaFluor-555–conjugated phalloidin. (D) α-tubulin labeling with anti–α-tubulin antibody. Images were captured by a laser-scanning confocal microscope (Bio-Rad MRC-1024 ES) mounted on a Nikon TE-300 using a 60×/1.4 NA oil Plan Apo VC objective, digitally acquired using Laser Sharp software Version 3.2 (Bio-Rad), and analyzed using NIH ImageJ Version 1.42l software. Data are representative of 3 independent experiments. The GFP vector alone was used as a control. Ovals and arrows indicate transfected and nontransfected cells, respectively.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal