Kinetics of immune responses. (A) Kinetics of circulating idiotype-reactive cells in responding patients as measured by IFN-γ ELISpot. (B) Kinetics of anti-idiotype IgG titers in responding patients as measured by enzyme-linked immunosorbent assay. (C) PBMC proliferation in groups A (left) and B (right) induced by autologous dendritic cells as determined by flow cytometry measurement of carboxyfluorescein succinimidyl ester loss. (D) Analysis of CD4⁺ and CD8⁺ lymphocyte subsets in proliferating cells after Fab^Id stimulation by flow cytometry in a representative patient. (E) Kinetics of the proportion of CD4⁺CD25⁺FoxP3⁺ regulatory T cells in peripheral blood lymphocytes during the vaccination period. (F) Prevaccination and postvaccination release of T helper 1 (IFN-γ and tumor necrosis factor-α) and T helper 2 cytokines (interleukin-4 [IL4] and interleukin-5 [IL5]) by peripheral blood lymphocytes on Fab^Id stimulation.