Iron deposition in liver and spleen in Alk2^fl/fl, Alk2^fl/fl; Alb-Cre, Alk3^fl/fl and Alk3^fl/fl; Alb-Cre mice. Formalin-fixed, paraffin-embedded liver (A-D) and spleen (E-H) sections prepared from Alk2^fl/fl, Alk2^fl/fl; Alb-Cre, Alk3^fl/fl, and Alk3^fl/fl; Alb-Cre mice were stained with Prussian blue and diaminobenzidine (A-B) or Prussian blue alone (C-H). Sections were prepared from at least 4 animals in each group; representative photomicrographs are shown. Scale bars represent 100 μm.