Hepatic hepcidin, transferrin receptor 1 (Tfrc), Bmp6, and Id1 mRNA levels are decreased in Alk3^fl/fl; Alb-Cre mice at the age of 12 weeks. (A) Hepatic hepcidin mRNA levels were determined by quantitative RT-PCR in Alk2^fl/fl, Alk2^fl/fl; Alb-Cre, Alk3^fl/fl, and Alk3^fl/fl; Alb-Cre mice (n ≥ 7 per group). *P = .03, Alk2^fl/fl; Alb-Cre versus Alk2^fl/fl. ^#P = .02, Alk3^fl/fl; Alb-Cre versus Alk3^fl/fl. (B) Hepatic Tfrc (n ≥ 7 per group). *P = .04, Alk3^fl/fl; Alb-Cre versus Alk3^fl/fl. (C) BMP6 (n ≥ 7 per group). *P = .049, Alk3^fl/fl; Alb-Cre versus Alk3^fl/fl. (D) Id1 (n ≥ 7 per group). *P = .04, Alk3^fl/fl; Alb-Cre versus Alk3^fl/fl. mRNA levels were also measured.