Expression profiles of hepcidin stimuli and suppressors in isolated rat liver cells. The mRNA levels of BMP6 (A), TMPRSS6 (B), BMP2 (C), BMP4 (D), BMP5 (E), BMP9 (F), and TGF-β1 (G) in isolated rat liver hepatocytes (Hepat, n = 5), KCs (n = 7), SECs (n = 4), and HSCs (n = 6) were analyzed by qRT-PCR. The sequences of the primers used for the analysis are listed in Table 1. Results are expressed as the amounts relative to GAPDH. We previously showed that different rat liver cell populations have similar levels of GAPDH mRNA.²⁸  The mean values and the SD for each cell population are presented.