TWIST-1 expression in primary CD34⁺ and CD34⁻ selected CML cells. Gene expression in CD34 immuno-selected hematopoietic cells from CML patients. Results are expressed as a ratio to normal (healthy donors) CD34⁻ cell values. (A) Comparative expression of TWIST-1, BMI-1, and BCR-ABL in CD34⁺ () and CD34⁻ (■) compartments from CML samples (n = 40). (B) Comparative expression of TWIST-1 in CD34⁺ (left panel) and CD34⁻ (right panel) cells in controls (healthy BM donors; n = 10), in CP (n = 20), and in advanced-phase (n = 16) CML cells. Horizontal bars represent the medians. (C) Comparative expression of TWIST-1, in CD34⁺ (left panel) and CD34⁻ (right panel) cells in CML patients responsive (Resp; n = 18) or nonresponsive (Non Resp; n = 25) to imatinib. Horizontal bars represent the medians. (D) Mean TWIST-1 expression in CD34⁺ () or CD34⁻ (■) CML cells from a total of 32 patients at diagnosis before any treatment. P1 to P11 indicate individual data from patient 1 (P1) to patient 11 (P11). Their mutational status (NM indicates nonmutated; and M, mutated) or presence of chromosome Ph duplication (Dup Phi) status are indicated below the graph. ND indicates not done; and UD, undetected.