The pCD11c^1500bp drives expression selectively in DCs. (A) Schematic representation of the lentiviral-based SIN-vector containing a fragment of the mouse CD11c promoter to control expression of enhanced GFP (eGFP). CMV indicates cytomegalovirus; Ψ, packaging signal; SIN, self-inactivating; LTR, long terminal repeat; WPRE, woodchuck hepatitis virus post-transcriptional regulatory element; and ΔU3, deletion in the U3 region. (B) Analysis of the CD11c^1500bp promoter activity in different organs. HSCs from C57BL/6 mice were modified by transduction with a lentiviral vector presented in panel A and injected into lethally irradiated C57BL/6 recipient mice. After 8 weeks, single-cell suspensions of spleen, thymus, and lymph nodes (mandibular, axillary, and subiliac) from these chimeras were stained for CD11c and MHC-II, and GFP expression was analyzed by flow cytometry. For detailed quantification, we gated on CD11c^hi and CD11c^lo cells and histograms were shown. Data are representative of 2 independently performed experiments with 4 or 5 mice per group.