Sequential deletion analysis of the CD11c promoter in vivo. (A) Schematic illustration of pCD11c deletion constructs with the respective localization of the CD11c/DC-STAMP promoter model. (B) Promoter activity of different sizes of the mouse CD11c promoter measured by flow cytometry. Spleen cells from bone marrow chimeras generated with HSCs transduced with lentiviral constructs carrying the indicated pCD11c segment were analyzed for GFP expression. The different cell types were identified based on the expression of the indicated markers. Overlays were generated after gating on the relevant population using cells from C57BL/6 mice as negative controls. For every promoter construct, the data shown are representative of 2 independently performed experiments with 4 or 5 mice per group.