Expression of Ppef2 and Pftk1 in DCs. (A) Gene expression profiling of ex vivo spleen cells. After density enrichment, DCs were sorted on a BD FACSAria instrument based on their expression of CD11c, MHCII, and CD8. CD8 T cells were identified as CD3⁺, CD8⁺, and CD4⁻ cells. Gene expression levels were calculated relative to ubiquitin C. Error bars represent SD between 2 independently performed experiments. (B) Gene expression profiling of DCs generated in vitro. Real-time PCR analysis of RNA from samples isolated at days 0, 4, 7, and 8 during DC culture. At day 7, parts of the cultures were stimulated overnight with LPS (2 μg/mL). Shown is relative gene expression after normalization to ubiquitin C. Data are representative of 2 independently performed experiments. nd indicates not detectable.