The effects of Y27632, 8-Br-cAMP, and TS2/16 on cell-matrix adhesion, cell-cell adhesion, and stress fiber formation. HMEC transductants were treated with Y27632 (10μM), 8-Br-cAMP (500μM), or TS2/16 (1 μg/mL) overnight either before (A) or during (B-C) experiments. (A) The 35-minute, static cell-matrix adhesion assay was performed on LN 332 (1 μg/mL) as described in supplemental Methods. *P < .01. **P < .05. (B) Cell-cell adhesion assay was performed in complete media. *P < .01. **P < .05. (C) After ECs were spread overnight in the presence or absence of treatment, the IF staining of F-actin was performed as described in supplemental Methods. Images were taken on a Zeiss LSM510 confocal fluorescence microscope under a 100×/1.4 NA oil objective. Bar represents 10 μm. See supplemental Figure 6 for the codistribution of F-actin and vinculin.