Flow cytometry of HSP105 in malignant lymphoid cell lines and normal cells. Surface (A) and intracellular (B) flow cytometric analyses of normal B cells, (1) low-grade B-cell NHL cell lines DOHH-2, (2) SC-1, (3) SU-DHL-6, and (4) KARPAS-422, (5) high-grade B-cell NHL cell lines RL-19, (6) SU-DHL-4, (7) RAJI, (8) NAMALWA, and (9) GRANTA-519, (10) Hodgkin lymphoma cell lines L-540 (11) and HDMYZ, (12) multiple myeloma cell line KMS-11, (13) normal T cells, (14) malignant T cell lines MOLT-4 (15), and SU-DHL-1 (16) stained with rabbit polyclonal antihuman HSP105 Ab and FITC-labeled antirabbit IgG. HSP105 relative MFI, calculated as the ratio between stained samples and negative control MFI values (left panels), and percentage of HSP105⁺ cells (right panels). Data are mean ± SE of 6 independent experiments.