Antitumor effects of HSP105 targeting in the NAMALWA model. (A) IHC of NAMALWA xenografts from control rabbit IgG- (top) and anti-HSP105 Ab-treated (bottom) animals. Representative hematoxylin and eosin (i), Granzyme B (ii), and CD56 (iii) stainings. Digital images were acquired with Leica DFC320 digital camera and Leica DM2000 microscope, and processed using Photoshop CS4 software version for Macintosh Pro personal computer. Original magnifications: i, ×200; ii,iii, ×400; and insets, ×400. (B) Absolute number of Granzyme B⁺ (i) and CD56⁺ (ii) cells in sections from control rabbit IgG- and anti-HSP105 Ab-treated xenografts. Immunoreactive cells (ie, yellow/brown-stained cells) were counted in 5 high-power microscopic fields (original magnification, ×400) for each condition, and the result was expressed as an average. The boxes extend from the 25th to the 75th percentiles, the lines indicate the median values, and the whiskers indicate the range of values. Results from 2 independent experiments are shown. Statistically significant differences between groups were assessed with the unpaired 2-tailed Student t test: ***P < .001.