PKD expression and activation profile in platelets. Washed platelets from WT, PKD2^−/− (A), or PKD2^SSAA/SSAA (B) mice (2 × 10⁸/mL) were incubated with CRP (5 μg/mL) or thrombin (1 unit/mL) for 1 minute under stirring conditions. Platelets were then solubilized in Laemmli sample buffer and whole cell lysates were subjected to SDS-PAGE, transferred to PVDF membrane, and immunoblotted with PKD1/2, PKD3, P-PKD (Ser916), or P-PKD (Ser744) antibodies. Data shown are representative of 3 independent experiments.