Oncogenic Nras initiates TALL in a dose-dependent manner. Lethally irradiated mice (CD45.1+) were transplanted with 2.5 × 105 total bone marrow cells of control, Nras G12D/+, or Nras G12D/G12D mice along with same number of competitor cells. (A) Kaplan-Meier survival curves of reconstituted mice. Cumulative survival was plotted against days after transplantation. (B) Disease distribution patterns in recipient mice transplanted with Nras G12D/+ or Nras G12D/G12D cells. (C) Hepatosplenomegaly and enlarged thymi in TALL-het and -homo mice. (Top) Enlarged thymus in a representative recipient mouse that developed a TALL disease. (Bottom) Results are presented as averages of spleen, liver, or thymus weights + SDs. Student t test was performed: *P < .001. (D) Representative histologic H&E sections of thymus from control, TALL-het, and TALL-homo mice. (E) Flow cytometric analysis of total thymocytes of representative control, TALL-het, and TALL-homo mice.
