ICAM-1 and ICAM-3 regulate the neutrophil-dependent potentiation of IL-12p70 and IFNγ release by slanDCs and NK cells, respectively. slanDCs were cultured alone or with neutrophils and then stimulated with LPS + IFNγ in the absence (A-C) or presence of αICAM-1 (A), αICAM-2 (B), and αICAM-3 (C) mAbs for 18 hours. Extracellular IL-12p70 was then measured in culture supernatants (n = 4). NK cell/slanDC cocultures were incubated either alone or with neutrophils and then treated with LPS + IL-15/IL-18 in the absence (D-F) or presence of αICAM-1 (D; n = 5), αICAM-2 (E; n = 3), and αICAM-3 (F; n = 9) mAbs. After 18 hours, both IFNγ and IL-12p70 were measured in culture supernatants using ELISA. (A-F) ***P < .001, **P < .01, and *P < .05 by 1-way ANOVA of paired samples.