Colocalization of CD18 with ICAM-1 and with ICAM-3 in the contact regions of neutrophils/slanDCs and neutrophils/NK cells, respectively. (A-B) Neutrophil/NK cell/slanDC cocultures were incubated with LPS + IL-15/IL-18 prior to preparing the samples for confocal microscopy analysis as described in “Laser confocal microscopy.” The contact regions between neutrophils and slanDCs (A) and neutrophils and NK cells (B) are shown by bright-field images (BF; left panels). Overlay of fluorescent images (middle and right panels) demonstrate the colocalization of (A) CD18 (green) and ICAM-1 (red) and of (B) CD18 (green) and ICAM-3 (red) in the contact regions between neutrophils and slanDCs, and neutrophils and NK cells, respectively. Representative images from 3 experiments for each condition are shown.