CD19-deficient, Notch2IC-expressing B cells show increased phosphorylation of Akt and regulation of Akt downstream targets. (A) Graphs show mean values with SDs of the fold induction of indicated phosphorylated and nonphosphorylated proteins in relation to GSK3β, determined by densitometry of Western blots. Values from Notch2IC//CD19Cre^+/− mice are displayed as n-fold of values from CD19Cre^+/− control mice, which were set to 1. Analogously, values from Notch2IC//CD19Cre^+/+ mice are displayed as n-fold of values from CD19Cre^+/+ control mice, which were set to 1. Mean values were calculated from 4 independent experiments. (B) In comparison to control B cells, Foxo1 levels are decreased in Notch2IC-expressing B cells. Splenic B cells were purified from mice with indicated genotypes, and whole-cell extracts from unstimulated cells were subjected to immunoblot analysis with the use of antibodies specific for Foxo1. Equal protein loading was controlled by α-tubulin staining. The result is representative for 4 independent experiments.